Coding
Part:BBa_K5117003:Design
Designed by: Jenny Sauermann, Lilli Kratzer, Katrin Lehmann Group: iGEM24_TU-Dresden (2024-08-31)
AtCelA
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
removal of restriction sites which are used for RFC[10] or Type IIS standards,
exchange of start codon (GTG > ATG) for usage in Bacillus subtilis as host organism
Source
Acetivibrio thermocellus